60-Day Assessment of Mr. Flush® Cleanser on Hematological indices and Kidney Histoarchitecture in Exposed Wistar Rats

Abstract

ct Aim: there is a surge in herbal product sales with less adequate information on their safety. The toxicity concerns of Mr. Flush® polyherbal formulation on hematological and biochemical parameters was investigated in Wistar rats. Methodology: Thirty Wistar rats of both genders were randomly allotted to six groups (5 per group) and treated by oral administration daily thus: Groups 1 and 2-Controls (distilled water, 10 mL/kg), Groups 3-4; 5-6 received the Polyherbal mixture (374, 748) mg/kg, respectively. On 62nd day, animals were euthanized under diethyl ether anesthesia and sacrificed. Blood samples were collected using cardiac puncture for biochemical evaluation. Eviscerated liver and kidneys were weighed and fixed in 10% formalin for histopathological examination. Results: The LD50 of the Polyherbal Formulation was estimated to be 3740.17 mg/kg (mouse per oral). Results were considered significant at p ≤0.05. There was significant increase in blood platelets (PLT) in low dosed and high dosed groups compared with control, WBCs increased in treatment groups compared with controls. Kidney function parameters such as Urea and Creatinine were elevated in all treatment groups compared to control. Histopathology of the kidneys revealed degrees of pathologies such as occluding Bowman’s space, widened proximal and distal convoluted tubules, hyperplasia of cortical tissue cells as well as hyperplasia of tubular and connective tissue cells