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dc.contributor.authorIHEKAIKE, Marcia Morayo-
dc.date.accessioned2024-06-18T18:37:01Z-
dc.date.available2024-06-18T18:37:01Z-
dc.date.issued2020-
dc.identifier.citationEbonyi AO, Oguche S, Abok II, Isa YO, Ani CC, Akhiwu HO, Ihekaike MM, Yiltok ES, Ochoga MO, Sagay AS. (2020) Improving the diagnosis of pulmonary tuberculosis using line probe assay and determining the factors associated with the disease in children in Jos, Nigeria. Germs. 2020 Dec 28;10(4):328-337en_US
dc.identifier.urihttp://localhost:8080/xmlui/handle/123456789/2115-
dc.description.abstractIntroduction Diagnosing tuberculosis (TB), including pulmonary tuberculosis (PTB), in children remains a challenge, partly due to its paucibacillary nature in young children. Data on the use of line probe assay (LPA), on gastric and sputum samples, for diagnosing PTB in children are scarce. We determined the proportion of samples positive for Mycobacterium tuberculosis (MTB) by smear microscopy (SM) and LPA in presumptive PTB cases as well as the factors associated with PTB confirmed by LPA, in children in Jos, Nigeria. Methods An observational study in children aged 6 months-16 years. Gastric and sputum samples were examined by SM and by LPA for MTB using GenoType MTBDRplus Ver 2.0 (Hain Lifescience). Multivariate logistic regression was performed to determine the factors associated with PTB. Results Out of 103 children with presumptive PTB, 47 had confirmed PTB, 26 unconfirmed PTB and 30 unlikely PTB by LPA. In 67 gastric samples, MTB was identified by SM in 2 (3.0%) compared to 28 (41.8%) by LPA while in 31 sputum samples, MTB was identified by SM in 5 (16.1%) compared to 18 (58.1%) by LPA. The factors associated with pulmonary tuberculosis were an abnormal chest X-ray (adjusted odds ratio (AOR))=12.39 [3.75-40.90], p<0.001), sleeping in the same room with more than three persons (AOR=3.30 [1.23-8.85], p=0.018) and sleeping in a room with none or one window (AOR=2.86 [1.03-7.95], p=0.044). Conclusions Line probe assay improves the diagnosis of pulmonary TB in children, especially with gastric samples, while an abnormal chest X-ray is a useful adjunct in PTB diagnosis. Avoiding overcrowding and having windows in sleeping rooms are a necessary part of TB prevention.en_US
dc.description.sponsorshipThe Medical Education Partnership Initiative in Nigeria (MEPIN) provided support for this study, and MEPIN was in turn funded by Fogarty International Center, the Office of AIDS Research, and the National Human Genome Research Institute of the National Institute of Health, The Health Resources and Services Administration (HRSA) and the Office of the US Global AIDS Coordinator under Award Number R24TW008878. Supported was also provided by the Fogarty International Center (FIC), Office of the Director (OD/NIH), National Institute of Neurological Disorders and Stroke (NINDS/NIH) and the National Institute of Nursing Research (NINR/NIH) of the National Institutes of Health under Award Number D43 TW010130en_US
dc.publisherGermsen_US
dc.subjectLine probe assay, smear microscopy, gastric sample, sputum sample, pulmonary tuberculosis, chest X-ray, childrenen_US
dc.titleImproving the diagnosis of pulmonary tuberculosis using line probe assay and determining the factors associated with the disease in children in Jos, Nigeria.en_US
dc.typeArticleen_US
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