Abstract:
Aims: This work aimed to evaluate the antimicrobial potential of the stem bark extracts of
P. biglobosa on selected bacteria species, Escherichia coli, and Staphylococcus aureus.
Place and Duration of Study: Microbiology laboratory, Department of Biological Sciences,
Bingham University-Karu, Nasarawa State, Nigeria.
Methodology: Plant extracts were made by boiling, soaking and methanol extraction, and the
antimicrobial assay was performed using the disc and agar-well diffusion methods.
Results: The results indicated all the extract exhibited antimicrobial properties. The highest
potential activity was observed with the macerated extract with zones of inhibition at 12.5 mm and
6.5 mm against S. aureus and E. coli, respectively in the agar-well diffusion method while no active
zone of inhibition was recorded in the disk diffusion method. It was noted that the macerated extract
showed the highest MIC of 100 mg/ml with a zone of inhibition at 15.0 mm against S. aureus.
Original Research Article
Ihuma et al.; Asian J. Biol., vol. 16, no. 3, pp. 1-9, 2022; Article no.AJOB.92799
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Conclusion: The results obtained showed that the macerated extract of the plant possess the
highest antimicrobial effect against S. aureus, and E. coli. Although observed potency of this extract
in vitro, may not be translated to the same activity in vivo. However, the results confirmed the
efficacy of the plant as a natural antimicrobial and can be further investigated for possible
antimicrobial drug discovery.
