Abstract:
Background: Infertility is a growing gynaecological problem in couples of childbearing age having difficulties
bearing children. Couples with infertility are known to present with high levels of stress and psychopathology A cross
sectional case control study aimed at evaluating the hormonal profile, some stress biomarkers, sperm analysis in
infertile couples was carried out to ascertain their contributions to infertility in couples of child bearing age.
Methods: Serum Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH), Prolactin, Progesterone, Estrogen
(E2), Testosterone, Salivary Cortisol and Salivary Alpha Amylase were evaluated using both competitive and noncompetitive Enzyme Linked Immunosorbent Assay (ELISA) techniques; while sperm cells analysis were evaluated
using conventional methods, in 164 infertile couples (study) and 100 fertile couples (control) attending fertility clinic
in General Hospitals in Abuja, Nigeria.
Results: The mean serum Prolactin and Salivary (S) Cortisol in the female were significantly higher (p<0.05) in the
study group relative to the control group, while Salivary Alpha Amylase and LH show no significant difference
(p>0.05) between the two groups. FSH, Progesterone and E2 in the female were significantly lower in the study group
(p<0.05) compared with control group. Testosterone, FSH, Sperm cells count and Sperm activity (%) were
significantly lower (p<0.05) in the male study group relative to the control group; while prolactin, S. cortisol and S.
amylase were significantly higher (p<0.05) in the male study group relative to the control group.
Conclusions: Abnormal hormones values and abnormal sperm quality and quantity are associated with elevated stress
biomarkers in couples presenting with infertility. Strong positive correlations exist between hormones and stress
biomarker in infertility conditions.
