Effects of ethanol extract of Dissotis rutundifolia on the histology of the ovary, uterus and Gonadotropins of adult female Whistar rats.

Abstract

This study was aimed at evaluating the effects of ethanol extract of Dissotis rutundifolia on the histology of the ovary and uterus of Adult whistar rats. A total of twenty (20) wister rats weighing 150- 180g were used in this experiment and were divided into 5 groups (1-5) of 4 rats per group. Group 1(control) were administered 2ml distill water, group 2 (low dose) received 25mg/kg bw daily, group 3 (low dose + withdrawal) 25mg/kgbw, group 4(high dose) received 100mg/kgbw while group 5 received (high dose + withdrawal received) 100mg/kgbw. the extracts was administered by gavage once daily for 14 days in all treated groups while in group 3 and 5 there was withdrawal for seven days. At the end of treatment the rats were sacrificed and the Ovary and Uterus harvested and histologically processed and stained using H&E stains while blood samples was obtained intraoccularly for hormonal assay all groups. The histological results showed a loss in follicles production and an increase in atretic follicles in ovaries in ovaries with loss of endometrial and glandular cells in the uterus. There was a significant decrease in serum levels of follicle stimulating hormone (FSH) and Luteinizing hormones (LH) with prolonged dioestrous stage as compared to the normal control group. In the withdrawal groups normal ostrous cycle was observed with production of ovarian primary follicles, restoration of uterine endometrium and glandular cells. The level of FSH and LH was also restored.