Abstract:
Safety or
toxicity
assessment of
newly
discovered
endophytes
with potential
therapeutic
applications is
very crucial prior
to their approval
for human use.
This is to
ascertain the
possible
undesirable
outcomes of the
drug agents
within a shortterm of 24 h of
administration
(acute toxicity).
This study was
TIMBOU-AFRICA ACADEMIC PUBLICATIONS
INTERNATIONAL JOURNAL, NOVEMBER, 2023 EDITIONS
VOL. 15 NO. 3 ISSN: 2373-1340
INTERNATIONAL
JOUNAL OF:
SOCIAL HEALTH AND
MEDICAL RESEARCH
TIMBOU-AFRICA ACADEMIC PUBLICATIONS
NOVEMBER, 2023 EDITIONS, INTERNATIONAL JOURNAL OF:
TIJSHMR
2
SOCIAL HEALTH AND MEDICAL RESEARCH VOL. 15
ISSN: 2373-1340
roots, fruits, seeds, barks as well as the flowers could be useful as both
traditional and allopathic medicines (Abiole,2022). Mitracarpus scaber
aimed at determining the adverse effects that could be observed within
a short-term of 24 h of oral administration of multiply doses of metabolic
ethyl acetate extracts of leaf parts of Mitracarpus scaber to rodents. In
determining the median lethal dose (LD50) of the crude extract, a
method described by Lork was followed, which involves two phases of
administration of test drug to experiment animals. In the first phase, oral
gavage needle in a single dose disposable syringes were used to
administer three dose levels (10, 100, and 1000 mg/kg) of ethyl acetate
extract to mice of three in a group. Under critical observation, behavioral
changes and other possible signs of death/adverse effects associated
with the test extract were documented. The second phase was a
deduction of first phase, eight mice were divided into four groups of two
mice each. Each group was treated orally with graded doses of 1200,
1600, 2900, 5000 mg/kg respectively. Signs of adverse drug reactions
and other side effects within 24 hours were observed and recorded and
from the results gotten, final LD50 was evaluated. Results from the
treatment showed that the animals were not subjected to any
immediate risks of drug reaction or adverse effects on 24 h exposure and
the LD50 for extract was > 5000 mg/kg. Also, on completion of the
treatment some parameters evaluated showed significant differences
when compared with standards. Generally, the result of this study shows
that the metabolic crude extract of endophytic fungi isolated from
Mitracarpus scaber showed no oral acute toxicity in mice. However,
further evaluation of sub-acute and long-term toxicity studies using oral
and other routes of administration is recommended.
